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However, Nakamoto has never revealed any personal information about himself and his identity has never been confirmed. This has led many to believe the name is a pseudonym for one or more creators. In 2009, Bitcoin was first used after it was released as open-source software, when Nakamoto mined the starting block of the blockchain. This is referred to as the Genesis Block, and it contained the first 50 Bitcoins ever created. From there on, Bitcoin continued to be mined by other early contributors until 2010. That’s when programmer Laszlo Hanyecz made the first known commercial transaction using the cryptocurrency through the purchase of two Papa John’s pizzas for 10,000 Bitcoins. (Fun fact: That amount of Bitcoin is worth over $300 million as of this writing). Since then, Bitcoin has been traded hundreds of millions of times, with the earliest major transactions occurring in black markets. 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The isolated bacterial colonies are examined by staining or plate counting and then identified using biochemical techniques. However, the culture method is disadvantageous in terms of not only relatively long test time but also high costs. Accordingly, development of a more sensitive bacterial detection system is urgently demanded. In recent years, biosensors have been applied to medical fields because of their advantages, such as specific/highly sensitive detection, simple operation, portability, compactness, low cost, etc. Biosensors provide a label-free and real-time sensing capability without requiring an amplification step. By using bacteria, enzymes and specific antibodies as sensing elements, biosensors have been developed. Among biosensors, an electrochemical biosensor detects electron signals generated at redox reactions between an electron carrier (e.g., ferricyanide, ferrocyanide or ferricyanide/ferricyanide complex) and the electron carrier oxidized by an electron accepting substance (e.g., electron acceptor) oxidized by an electron receiving substance (e.g., electron receiving substance) electrochemically or photochemically, and then recognizes the electron carrier oxidized by the electron receiving substance as a reaction product. The electrochemical biosensor attracts attention in the medical field, and many studies have been conducted to develop an electrochemical bacterial biosensor based on the principle. U.S. Pat. Nos. 6,544,694, 5,820,443, 6,852,221 and 6,844,572 and U.S. Patent Application Publication Nos. 2005-266664, 2005-264847 and 2006-015410 disclose various electrochemical bacterial biosensors for selectively detecting individual bacteria. 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